In vitro Study of SARS Virus Infection and Cell Migration
Date Issued
2004
Date
2004
Author(s)
伍安怡
DOI
923112B002039
Abstract
To investigate the immunoapthogenesis of SARS, we infected a number of
human cell lines with SARS-CoV. By immunoflourescent staining with sera from
SARS patients, we identified one monocytic cell line that is susceptible to the virus.
Total RNA was extracted from the cells to assay for the expression of chemokines.
Results of Multi-Probe RNase protection assay demonstrated that the monocytic cell
line expressed a panel of chemokines after SARS-CoV infection, while the epithelial
cell line expressed only one. In addition, SARS-CoV-infected epithelial cell expressed
adhesion molecules. Western blot analysis showed that the monocytic as well as the
epithelial cells express ACE-2, a putative SARS-CoV receptor. Comparing DC-SIGN
transfected cells to their parental cell line; we demonstrated that expression of
DC-SIGN did not change the kinetics of chemokines induced by SARS-CoV.
Based on our data, we concluded that both human epithelial cells and monocytic
cells are hosts for SARS-CoV. The possible scenario of severe acute respiratory
distress that occurs in patients infected by SARS-CoV is as follows :( 1) SARS-CoV
infects pulmonary epithelial cells; (2) The infected epithelial cells express adhesion
molecules and produce chemokine to attract monocytes; (3) The recruited monocytes
in turn are infected by SARS-CoV to produce a panel of chemokines; (4) These
chemokines are important in recruiting T cells, neutrophils and more monocytes into
the lungs, which result in tissue damage and eventual respiratory distress.
Publisher
臺北市：國立臺灣大學醫學院免疫學研究所
Type
journal article
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