EGb761 inhibits inflammatory responses in human chondrocytes and shows chondroprotection in osteoarthritic rat knee
Journal
Journal of Orthopaedic Research
Journal Volume
31
Journal Issue
7
Pages
1032-1038
Date Issued
2013
Author(s)
Chen Y.J.
Chiu C.Y.
Lin T.H.
Chen C.F.
Abstract
Osteoarthritis (OA) is a degenerative joint disease involving a combination of cartilage degradation and inflammation. EGb761, a standardized extract of Ginkgo biloba leaves, holds an anti-inflammatory potency. Here, we determined whether EGb761 could inhibit lipopolysaccharide (LPS)- and IL-1β-induced inflammatory responses in human articular chondrocytes and apply the chondroprotection in OA rats. We found that LPS markedly induced the productions of PGE2 and NO and the protein expressions of COX-2 and iNOS in human chondrocytes. LPS was also seen to up-regulate the expressions of toll-like receptor-4 (TLR4), its downstream signal TNF receptor-associated factor 6 (TRAF6), and nuclear factor (NF)-κB signaling. These LPS-induced inflammatory responses were efficaciously reversed by EGb761 and its active components quercetin and kampferol. The similar results could be observed by using IL-1β as an in vitro model to mimic an inflammatory response. In an OA rat model, PGE2 and NO levels in blood, the histological alterations, and COX-2 and nitrotyrosine expressions in cartilages were markedly increased, which were effectively reversed by EGb761. Our results suggested that EGb761 exerts the anti-inflammatory effects on human articular chondrocytes and OA rats. ? 2013 Orthopaedic Research Society.
SDGs
Other Subjects
3 nitrotyrosine; antiinflammatory agent; collagenase 3; cyclooxygenase 2; Escherichia coli lipopolysaccharide; Ginkgo biloba extract; I kappa B alpha; inducible nitric oxide synthase; interleukin 1beta; interleukin 6; nitric oxide; prostaglandin E2; quercetin; toll like receptor 2; toll like receptor 4; transcription factor RelA; tumor necrosis factor receptor associated factor 6; adult; aged; animal experiment; animal model; antiinflammatory activity; article; cartilage cell; cell stimulation; chondroprotection; clinical article; controlled study; cytokine production; histology; human; human cell; human tissue; in vitro study; knee osteoarthritis; male; nonhuman; priority journal; prostaglandin synthesis; protein expression; protein phosphorylation; rat; signal transduction; upregulation; Aged; Animals; Cells, Cultured; Chondrocytes; Cyclooxygenase 2; Dinoprostone; Disease Models, Animal; Humans; Interleukin-1beta; Lipopolysaccharides; Male; Middle Aged; NF-kappa B; Nitric Oxide; Nitric Oxide Synthase Type II; Osteoarthritis, Knee; Plant Extracts; Rats; Rats, Wistar; Toll-Like Receptor 4; Tyrosine