The metabolome profiling and pathway analysis in metabolic healthy and abnormal obesity
Journal
International Journal of Obesity
Journal Volume
39
Journal Issue
8
Pages
1241-1248
Date Issued
2015
Author(s)
Chen H.-H.
Wang S.-Y.
Tsai Y.-S.
Chang C.-S.
Kuo T.-C.
Yao W.-J.
Shieh C.-C.
Wu C.-H.
Abstract
Objectives:Mechanisms of the development of abnormal metabolic phenotypes among obese population are not yet clear. In this study, we aimed to screen metabolomes of both healthy and subjects with abnormal obesity to identify potential metabolic pathways that may regulate the different metabolic characteristics of obesity.Methods:We recruited subjects with body mass index (BMI) over 25 from the weight-loss clinic of a central hospital in Taiwan. Metabolic healthy obesity (MHO) is defined as without having any form of hyperglycemia, hypertension and dyslipidemia, while metabolic abnormal obesity (MAO) is defined as having one or more abnormal metabolic indexes. Serum-based metabolomic profiling using both liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry of 34 MHO and MAO individuals with matching age, sex and BMI was performed. Conditional logistic regression and partial least squares discriminant analysis were applied to identify significant metabolites between the two groups. Pathway enrichment and topology analyses were conducted to evaluate the regulated pathways.Results:A differential metabolite panel was identified to be significantly differed in MHO and MAO groups, including L-kynurenine, glycerophosphocholine (GPC), glycerol 1-phosphate, glycolic acid, tagatose, methyl palmitate and uric acid. Moreover, several metabolic pathways were relevant in distinguishing MHO from MAO groups, including fatty acid biosynthesis, phenylalanine metabolism, propanoate metabolism, and valine, leucine and isoleucine degradation.Conclusion:Different metabolomic profiles and metabolic pathways are important for distinguishing between MHO and MAO groups. We have identified and discussed the key metabolites and pathways that may prove important in the regulation of metabolic traits among the obese, which could provide useful clues to study the underlying mechanisms of the development of abnormal metabolic phenotypes. ? 2015 Macmillan Publishers Limited. All rights reserved.
SDGs
Other Subjects
glycerophosphate; glycerophosphorylcholine; glycolic acid; isoleucine; kynurenine; leucine; palmitic acid methyl ester; phenylalanine; tagatose; uric acid; valine; glucose blood level; adult; age; amino acid blood level; amino acid metabolism; Article; body mass; controlled study; degradation; dyslipidemia; fatty acid synthesis; female; human; hyperglycemia; hypertension; lipid blood level; liquid chromatography; major clinical study; male; mass fragmentography; mass spectrometry; metabolic abnormal obesity; metabolic healthy obesity; metabolic regulation; metabolome; obesity; phospholipid blood level; priority journal; sex; uric acid blood level; body fat distribution; glucose blood level; inflammation; metabolism; metabolome; metabolomics; obesity; pathophysiology; procedures; risk factor; Taiwan; Adult; Blood Glucose; Body Fat Distribution; Body Mass Index; Female; Humans; Inflammation; Male; Metabolic Networks and Pathways; Metabolome; Metabolomics; Obesity; Risk Factors; Taiwan
Publisher
Nature Publishing Group
Type
journal article