兩型斑馬魚肌肉調控因子，MRF4-I及MRF4-II，的分子結構、在胚胎早期發育的角色、轉錄調控與啟動子分析
Date Issued
2004
Date
2004
Author(s)
陳曜鴻
DOI
922321B002018
Abstract
The primary amino acids sequences of MRF4 are highly conserved with the
muscle regulatory factors (MRFs), but the molecular mechanism of MRF4 involved in
skeletal muscle cell specification and differentiation during late embryonic
development of fish is still unclear to date. In this report, we cloned the zebrafish mrf4
gene and showed that is located at the long arm of chromosome 4 (LG4) by
chromosome mapping. We also made the syntenic relationship of mrf4 gene between
zebrafish and human, and showed that zebrafish LG4 fragment between EST markers
fa05f06 and fk68a09 (including myf5) was related to human chromosome
12q13-12q21. We furthermore isolated two transcripts of mrf4 (ZMRF4-1 and
ZMRF4-2) which were result from alternative splicing. ZMRF4-1 possesses 206
amino acids whereas ZMRF4-2 possesses 239 amino acids. The ZMRF4-1 transcripts
were first detected at 14 h, and gradually increase until adult. In contrast, ZMRF4-2
transcripts were first detected from 6 h to adulthood. Both two types of mrf4 are
expressed at the hypaxial region of somites. Finally, we check the MRF4-morphants
phenotypes by using anti-myosin heavy chain antibody, and found that the myofibers
perform incorrect connection. Based on these observations, we proposed that MRF4 is
required for myofiber connection.
Subjects
myogenesis
transcription factor
alternative splicing
somite
zebrafish
Publisher
臺北市：國立臺灣大學分子與細胞生物學研究所
Type
report
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