Research and Development of Electrochemical Impedance Spectroscopy and Surface Plasmon Resonance Interferometer based Integrated System: use ATP-biological linkers to detect IFN-gamma for platform verifications
Date Issued
2016
Date
2016
Author(s)
Liu, Meng-Wei
Abstract
Tuberculosis is a highly contagious disease such that global latent patient can be as high as one third of the world population. Currently, latent tuberculosis was diagnosed by stimulating the T cells to produce the biomarker of tuberculosis, i.e., interferon-γ. In this thesis, we developed a paraboloidal mirror enabled surface plasmon resonance (SPR) interferometer that has the potential to also integrate ellipsometry, and interferometer to analyze the antibody and antigen reaction. To examine the feasibility of developing a platform for cross calibrating the performance and detection limit of various bio-detection techniques, electrochemical impedance spectroscopy (EIS) method was also implemented onto a biochip that can be incorporated into this newly developed platform. The microfluidic channel of the biochip was functionalized by coating the interferon-γ antibody so as to enhance the detection specificity. To facilitate the processing steps needed for using biochip to detect various antigen of vastly different concentrations, a kinematic mount was also developed to guarantee the biochip re-positioning accuracy whenever the biochip was removed and placed back for another round of detection. Before EIS can be utilized, SPR was also adopted to observe the real-time signals on the computer in order to analyze the success of each biochip processing steps such as functionalization, wash, etc. Finally, the EIS results and the optical signals obtained from the newly developed optical detection platform was cross-calibrated. Preliminary experimental results demonstrate the accuracy and performance of SPR and EIS measurement done at the newly integrated platform.
Subjects
Surface Plasmon Resonance
Electrochemical Impedance Spectroscopy
Kinematic Mount Design
Interferon-γ
cross-calibrating
SDGs
Type
thesis
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