Genomic heterogeneity in Klebsiella pneumoniae strains is associated with primary pyogenic liver abscess and metastatic infection
Journal
Journal of Infectious Diseases
Journal Volume
192
Journal Issue
1
Pages
117-128
Date Issued
2005
Author(s)
Abstract
Background. Primary pyogenic liver abscess (PLA) with septic complication by Klebsiella pneumoniae is an emerging infectious disease. Methods and results. Using DNA microarray hybridization, we identified a 20-kb chromosomal region that contained 15 open-reading frames (ORFs), including an iron-uptake system (kfu), a phosphoenolpyruvate sugar phosphotransferase system (PTS), and 6 unknown ORFs. The region was more prevalent among tissue-invasive strains (35/46) than among noninvasive strains (19/98) (P < .0001, χ2 test). To test the role played by this region in pathogenesis, 3 different deletion mutants (NTUH-K2044 [Δkfu], K2044 [ΔORF7-9], and K2044 [ΔPTS]) were constructed. Only the ΔkfuABC mutants showed decreased virulence in mice, compared with the wild-type strain. An in vitro assay confirmed the involvement of kfu in iron acquisition. There was a high correlation rate (85%) between the kfu/PTS region and 2 tissue invasion-associated chromosomal regions (allS and magA). Moreover, all 3 regions were present in strains that caused PLA plus endophthalmitis or meningitis. Conclusion. Our results suggest that chromosomal heterogeneity is present in tissue-invasive K. pneumoniae strains. A genotype containing all 3 regions is strongly associated with PLA and metastatic infection. These regions may serve as convenient markers for the rapid diagnosis of emergent tissue-invasive strains. ? 2005 by the Infectious Diseases Society of America. All rights reserved.
SDGs
Other Subjects
amino acid sequence; animal experiment; animal model; animal tissue; article; bacterial chromosome; bacterial meningitis; bacterial strain; bacterial virulence; colorimetry; controlled study; deletion mutant; DNA hybridization; DNA microarray; endophthalmitis; genetic heterogeneity; genotype; Gram negative sepsis; histopathology; human; in vitro study; iron transport; Klebsiella pneumoniae; LD 50; liver abscess; mouse; nonhuman; nucleotide sequence; open reading frame; operon; plasmid; polymerase chain reaction; priority journal; promoter region; slot blot hybridization; strain difference; survival rate; wild type; Animals; Bacterial Proteins; Chromosome Mapping; Endophthalmitis; Genome, Bacterial; Humans; Klebsiella Infections; Klebsiella pneumoniae; Liver Abscess, Pyogenic; Meningitis, Bacterial; Mice; Mice, Inbred BALB C; Microarray Analysis; Plasmids; Sepsis; Variation (Genetics); Virulence
Type
journal article