Creating Bioinert Surface by Grafting Poly(Sulfobetaine) on Polyelectrolyte Multilayers
Date Issued
2010
Date
2010
Author(s)
Tsai, Chih-Chi
Abstract
Nonfouling surface has been widely used in biomaterials due to its potential resistancy in non-specific adsorption. In our study, Sulfobetaine methacrylate (SBMA), with its zwitterionic character, was used to fabricate bioinert surfaces. P(SBMA)-COOH was fabricated by free radical polymerization, then it was grafted on PEI/PAA-AZ/PEI polyelectrolyte multilayer by carbodiimide reaction. Through this method, the surface modification of p(SBMA)-COOH can be easily prepared, and the use of polyelectrolytic method can also be applied onto various of substrates to inhibit cell adhesion.
Examinations were done on the p(SMBA)-COOH by altering its chain length, grafting pH value and p(SBMA)-COOH grafting concentration. The surface composition and the surface hydrophobicity were measured. As result, the surface became hydrophilic after grafting p(SBMA)-COOH at pH 3 and 6.5, with high content of sulfur. In contrast, there was no significant difference upon its hydrophobicity and content of sufler, before and after p(SBMA)-COOH grafting at pH 11 condition. In addition, pH 3 and 6.5 films exhibited resistance to protein adsorption, cell and platelets adhesion. Different substrates such as PDMS, PSF and PP were also used in fabricating similar modified films, and the cell adhesion resistance were observed on these p(SBMA)-modified substrates.
Since RGD-modified surfaces enhance cell adhesion, p(SBMA-SH)-COOH was fabricated by SBMA and 2-propene-l-thiol, and then grafted with RGD peptide for further analysis. As result, the cell density on RGD-modified surface was low.
These studies support the potential of our method for p(SBMA)-modified surface fabrication in order to obtain cell resistance. In addition, our method can also be applied on wide range of different substrates.
Subjects
bioinert
sulfobetaine methacrylate
cell adhesion
platelet adhesion
protein adsorption
Type
thesis
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