NADPH Oxidase 4 Mediates IL-1β induced CCL20 in Human Gingival Fibroblasts
Date Issued
2016
Date
2016
Author(s)
Huang, Lih-Jia
Abstract
Periodontal diseases have a high prevalence in Taiwan. The primary cause of periodontitis is poor or ineffective oral hygiene, which leads to the accumulation of bacterial matrix, called dental plaque. Subgingival micro-organisms would deposit endotoxin and cause further inflammation in the gum tissues and progressive bone loss. During the inflammation process, a lot of cytokines will increases in expression. One of them is Interleukin-1β (IL-1β). IL-1βhas the ability to induce Chemokine (C-C motif) ligand 20 (CCL20), and CCL20 can attract T helper cell to the inflamed gingival tissue, causing IL-17 to increase. Il-17 can promote osteoclast activity and subsequently escalate bone resorption. The overall mechanism involved in the induction of CCL20 by IL-1β is still unknown and it is the aim of our study to further investigate this signaling pathway. Previous studies had shown that IL-1βinduce CCL20 in Human gingival fibroblast (HGF) via a NF-κB dependent pathway. We discovered the NOX4 inhibitor, Plumbagin and ROS inhibitor, NAC can inhibit the above mentioned pathway. And IL-1β can induce NOX4 and ROS in HGF as well. This proved that the signaling pathway of CCL20 induction by IL-1βis NOX4 dependent. Furthermore, we also discovered that Curcumin has the ability to inhibit IL-1β from inducing NOX4 and CCL20 in HGF. This finding may be useful in developing future adjunctive therapy in treating periodontal disease.
Subjects
Periodontitis
Human gingival fibroblast
ROS
Curcumin
SDGs
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-105-R02422005-1.pdf
Size
23.32 KB
Format
Adobe PDF
Checksum
(MD5):cba82851413a701f3ba7917632d76b0d