The Isolation Rate, Molecular Typing, and Antimicrobial Susceptibility of Salmonella from Dog- and Cat-Food Products in Taiwan
Date Issued
2010
Date
2010
Author(s)
Chang, Chia-Yuan
Abstract
Salmonella-infected cats or dogs can shed the bacteria via feces which eventually causes human-salmonellosis. Several outbreaks worldwide have indicated that contact between human and dogs, cats, or pet foods may cause salmonellosis. There are no published surveys regarding Salmonella isolation from pet foods in Taiwan. In order to understand Salmonella contamination in pet foods and the antimicrobial susceptibility of the isolates, 413 pet food samples were collected, including 276 dog food and 137 cat food. These samples included normal dry feed, prescription diet, canned feed, and treats; the origins of these samples were sorted as locally produced or imported. The Salmonella isolation rate was 2.7% (11/413) from 11 samples with a total of 13 isolates found; no significant differences existed between any categories. The isolates were serotyped and S. Schwarzengrund was identified as the most prevalent serovar (53.9%), followed by S. Enteritidis (30.8%). Antimicrobial susceptibility of the isolates was determined via disk diffusion method. Ninety-three percent of the isolates were shown to have resistance to one or more antimicrobial agents, with the highest resistance rate to streptomycin (84.6%). The phylogenetic analysis was identified by pulsed field gel electrophoresis (PFGE) and random amplification of polymorphic DNA (RAPD) as an auxiliary tool. The isolates were indistinguishable by PFGE using six enzymes except one different S. Schwarzengrund isolate pattern. Only two different patterns of S. Schwarzengrund isolates by RAPD were found using primer P1254. The serotypes of the isolates from the pet food in Taiwan are potentially pathogenic to both humans and pets. Pets’ owners are at risk of exposure either by direct or indirect contact.
Subjects
Salmonella
pet food
antimicrobial susceptibility
PFGE
RAPD
Type
thesis
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