Response of prokaryotic communities to extreme precipitation events in an urban coastal lagoon: A case study of Yundang lagoon, China
Journal
Science of the Total Environment
Journal Volume
706
Date Issued
2020
Author(s)
Abstract
Increasing extreme precipitation events (EPEs) can induce biogeochemical disturbances in the coastal lagoon ecosystems. Very little is known about the response of prokaryotic communities to such influences, which are the key components mediating the biogeochemical cycling in lagoons. Here 16S rRNA gene amplicon sequencing and high-through quantitative PCR (HT-qPCR) were employed to investigate the distribution of prokaryotic communities and fecal indicator genes in the surface waters of Yundang lagoon, Xiamen, China during EPEs, respectively. Prokaryotic communities from rainwaters, influents (IFs) and effluents (EFs) from a nearby wastewater treatment plant were also characterised. The results indicated a significant variation in the composition of lagoon prokaryotic communities compared with rainwaters, IFs and EFs. Multivariate and phylogenetic signal analyses revealed that environmental filtering, mainly controlled by salinity, was the major ecological process responsible for the temporal succession of lagoon prokaryotic communities during EPEs. Moreover, the pollution indicator taxa (based on amplicon sequencing) and fecal indicator genes (based on HT-qPCR) demonstrated that EPEs may induce sewage overflows and fecal pollution (mainly from humans and dogs), resulting in an increase in the relative abundance of pollution indicator taxa and genes in Yundang lagoon. Network analysis illustrated that the number of network edges and keystone species decreased along the sampling times, implying that EPEs-induced disturbances may affect prokaryotic species associations. Taken together, this study provides an enhanced understanding of the responses of lagoon prokaryotic communities to EPEs-induced disturbances. © 2019 Elsevier B.V.
Subjects
Environmental filtering; Extreme precipitation events; Fecal pollution; Prokaryotic assemblages; Prokaryotic network; Yundang lagoon
Other Subjects
Biogeochemistry; Ecosystems; Effluents; Genes; Lakes; Polymerase chain reaction; Precipitation (meteorology); River pollution; RNA; Sewage; Wastewater treatment; Biogeochemical cycling; Extreme precipitation events; Fecal pollution; Pollution indicators; Prokaryotic assemblages; Prokaryotic species; Wastewater treatment plants; Yundang lagoons; Sewage lagoons; biogeochemical cycle; coastal lagoon; community composition; community response; extreme event; keystone species; precipitation (climatology); prokaryote; relative abundance; water pollution; amplicon; article; China; dog; feces; filtration; human; keystone species; lagoon; nonhuman; precipitation; real time polymerase chain reaction; salinity; sewage; waste water management; animal; China; ecosystem; phylogeny; prokaryotic cell; waste water; China; Fujian; Xiamen; Yundang Lagoon; Canis familiaris; Prokaryota; RNA 16S; Animals; China; Dogs; Ecosystem; Phylogeny; Prokaryotic Cells; RNA, Ribosomal, 16S; Waste Water
Type
journal article