Effective assay of bacterial transglycosylation by molecular turn-on sensing and a second-order scattering effect
Journal
Analyst
Journal Volume
146
Journal Issue
19
Pages
5843-5847
Date Issued
2021
Author(s)
Hsu T.-W
Abstract
Instead of using the lipid II substrate that requires prior labelling with a radioactive isotope or fluorophore to probe the formation of peptidoglycan in bacterial transglycosylation, the released undecaprenyl pyrophosphate (UPP) product is quantitatively measured either using a terpyridine-zinc fluorescence turn-on sensor or simply by the second-order scattering effect of the in situ formed UPP-calcium complex. Both the assay methods are utilized to identify moenomycin A as a potent transglycosylase inhibitor with a consistent IC50 value. This journal is © The Royal Society of Chemistry.
Other Subjects
Calcium compounds; Fluorophores; Assay methods; Calcium complexes; IC50 value; Labelings; Peptidoglycans; Scattering effects; Second order scattering; Terpyridines; trans-Glycosylation; Turn-on sensors; Glycosylation; peptidoglycan; bacterium; cell wall; Bacteria; Cell Wall; Peptidoglycan
Type
journal article