Use of Electric Field Manipulation in Protein Deposition for Highly-Sensitive Micro-Cantilever Biosensor
Date Issued
2005
Date
2005
Author(s)
Chen, Chien-Hsun
DOI
zh-TW
Abstract
It is becoming gradually obvious that the high-throughput recognition and quantification of bio-molecules are of great importances in biomedical detection and disease diagnosis. With the growing interest and fast development in bio-nanotechnology, bio-sensing tools have been moving towards miniaturization, high sensitivity, and great promise in low-cost as well. A novel electric field-manipulated biosensor of label-free biomolecular recognition based on the nano-mechanics transduction in a micro-fabricated cantilever has been developed. Use of inherent protein charge not in an isoelectric point state is proven to be driven in an electric field, commonly seen in electrophoreses processes. Several electric field-manipulated experiments were conducted between the electrodes of -10V, +10V, and no voltage applications. It was found that the sensing area of the electrode with -10 volt was collectively deposited with the net positive protein IgG1 in a buffer solution of pH ~ 5. The significant surface stresses arise in sensing area as a negative electrode, while most proteins are, in contrast, repelled out of the sensing area applied in positive electrode. With the protein isoelectric point characteristics, the attraction and repulsion of proteins have been demonstrated in manipulation under electric fields across the electrodes with the device sensing element. In protein attraction demonstration, the device is capable of highly sensitive antigen detection by collecting most antibodies in a far dilute concentration. In addition, both use of attraction and repulsion in manipulation show the potential of selective deposition and local repulsion in specific sensing surface among multiple disparate sensing agents.
Subjects
微懸臂樑生化感測器
電場操控
蛋白質佈植
micro-cantilever biosensor
electric field manipulation
protein deposition
Type
thesis
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