Lateral flow immunoassay with stacking effect for the high sensitive Staphylococcus aureus detection
Journal
22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Journal Volume
2
Pages
1057-1059
Date Issued
2018
Author(s)
Abstract
In this paper, we introduce an innovative “stacking pad” for an extended binding interaction of antigen and antibodies to enhance the detection sensitivity. The concept is adapted from a stacking gel of polyacrylamide gel electrophoresis (PAGE) which allows for proteins to stack in a concentrated area and expend the reaction time. We also examined various materials as the stacking pad to push the detection limit of the initial colorimetric signal. In order to verify the feasibility of the proposed platform, Protein A, a specific product of Staphylococcus aureus was chosen as a targeted biomarker. As a result, Protein A can be accurately visible at the 1 ng/mL detection limit in 20 min. Copyright ? (2018) by Chemical and Biological Microsystems Society. All rights reserved.
Subjects
Bacteria; Binding energy; Chemical detection; Electrophoresis; Proteins; Binding interaction; Detection limits; Detection sensitivity; Lateral flow immunoassay; Polyacrylamide gel electrophoresis; Protein A; Stacking effect; Staphylococcus aureus
Type
conference paper