Expression pattern and localization of adipsin in mouse mammary gland
Date Issued
2008
Date
2008
Author(s)
Huang, Pei-Yu
Abstract
The mouse mammary gland comprises alveoli and duct-like structure formed by mammary epithelial cells that are embedded in the mammary fat pad and is undergoing continuous structural and functinal changes. By using RNA suppression subtractive hybridization technique and dot blotting, we found an adipokine called adipsin had higher mRNA expression level during mamamry involution. Adipsin is known that functions in innate immunity and lipid metabolism. The aim of this study focused on exploring the expression pattern of adipsin in different stages of mouse mammary gland development and further prospect its functions in mammary gland. The expression of adipsin mRNA gradually decreased during pregnancy and lactation and gradually increased from involution day 3, and maintained a high level till day 14. A similar expression pattern of the adipsin protein was identified. By in situ hybridization and immunohistochemitry, adipsin mRNA and protein predominantly expressed in adipocytes and also in some collapsed alveoli clusters of epithelial. For in vitro and in vivo functional studies of adipsin in the future, an adenovirus-based expression system encoding a myc epitope-tagged mouse adipsin was established and could infect and express mouse adipsin abundantly in mouse epithelial cell line NMuMG. ur results indicated that adipsin may be one of the signals conveyed by fat pad, and play a role in the involution stage of mouse mammary gland. An adenovirus-expressed system of adipsin has been established for further functional studies of adipsin in mammary gland.
Subjects
mouse mammary gland
involution
adipocytes
mammary epithelial cells
adenovirus
Type
thesis
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