A new pepper disease in Taiwan caused by Tobacco mild green mosaic virus and its infectious cDNA clone construction
Date Issued
2005
Date
2005
Author(s)
Li, Ching-Yun
DOI
en-US
Abstract
Chili pepper (Capsicum annuum. L) belonging to the genus Capsicum in the family Solanaceae is a herbaceous plant that can be grown annually or a perennially (at tropic areas). Chili pepper was originated from Peru, South America and carried to the Europe by Christopher Columbus in the fifteenth century. Today, pepper is globally present and has becomes an important agricultural produce in Taiwan. Pingtung, Changhua, Nantou, Yunlin, and Chiayi counties are the main pepper growing districts in Taiwan. More than 25 viruses have been reported to infect pepper and have made virus disease one of limiting factors of pepper production. Virus-like yellowing and mild necrosis were observed on the chili pepper plants in the NTU experimental field. By the successive single-lesion isolation on Chenopodium amaranticolor, a viral isolate of a virus was obtained. Transmission electronic microscopy revealed numerous rigid rod-like particles about 300 nm long, that strongly suggesting that isolated virus was belong to the tobamovirus. According to the literature, tobamoviruses isolated from peppers in Taiwan included Pepper mild mottle virus (PMMoV), Tobacco mosaic virus (TMV), and Tomato mosaic virus (ToMV). To identify this unknown tobamovirus, first, molecular cloning this viral genome was conducted a poly (A) tail was added to the 3′ end of the purified viral RNA and the RACE strategy was perform to obtain independent clones and then sequenced. The result of blast search against the NCBI database indicated that the clones contained a partial sequence of Tobacco mild green mosaic virus (TMGMV). To our knowledge, this is the first report of TMGMV identified in Taiwan and this isolate was named as TMGMV-HP. To further cloning and characterization, the entire genome of the TMGMV-HP revealed that it was highly homologous to tobamovirus infecting solanaceous plants and showed 98% identity with the TMGMV-Japanese isolate. Also, a full-length cDNA clone of TMGMV was constructed and followed by successive infectivity tests, five infectious cDNA clones were obtained. An efficient TMGMV-based viral vector is still under construction. To determine the prevalence of TMGMV in Taiwan, samples collected from different sources were detected by ELISA and RT-PCR. The result suggested that mixed infection among TMGMV and other tobamoviruses occurred occasionally.
Subjects
菸草微綠嵌纹
病毒
TMGMV
Type
other
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