Photoswitchable carbon-dot liposomes mediate catalytic cascade reactions for amplified dynamic treatment of tumor cells
Journal
Journal of colloid and interface science
Journal Volume
628
Journal Issue
Pt A
Pages
717
Date Issued
2022-12-15
Author(s)
Lin, Yu-Feng
Lin, Yu-Syuan
Huang, Tzu-Yun
Wei, Shih-Chun
Wu, Ren-Siang
Huang, Chih-Ching
Huang, Yu-Fen
Abstract
Most biochemical reactions that occur in living organisms are catalyzed by a series of enzymes and proceed in a tightly controlled manner. The development of artificial enzyme cascades that resemble multienzyme complexes in nature is of current interest due to their potential in various applications. In this study, a nanozyme based on photoswitchable carbon-dot liposomes (CDsomes) was developed for use in programmable catalytic cascade reactions. These CDsomes prepared from triolein are amphiphilic and self-assemble into liposome-like structures in an aqueous environment. CDsomes feature excitation-dependent photoluminescence and, notably, can undergo reversible switching between a fluorescent on-state and nonfluorescent off-state under different wavelengths of light irradiation. This switching ability enables the CDsomes to exert photocatalytic oxidase- and peroxidase-like activities in their on- (bright) and off- (dark) states, respectively, resulting in the conversion of oxygen molecules into hydrogen peroxide (H2O2), followed by the generation of active hydroxyl radicals (OH). The two steps of oxygen activation can be precisely controlled in a sequential manner by photoirradiation at different wavelengths. Catalytic reversibility also enables the CDsomes to produce sufficient reactive oxygen species (ROS) to effectively kill tumor cells. Our results reveal that CDsomes is a promising photo-cycling nanozyme for precise tumor phototherapy through regulated programmable cascade reactions.
Subjects
Carbon-dot-liposomes; Cascade reactions; Enzyme mimics; Photocatalysts; Photodynamic therapy; Photoswitching
SDGs
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Type
journal article