Inhibitory effects of a rice hull constituent on tumor necrosis factor £\, prostaglandin E2, and cyclooxygenase-2 production in lipopolysaccharide-activated mouse macrophages
Journal
Annals of the New York Academy of Sciences
Journal Volume
1042
Pages
387-395
Date Issued
2005
Author(s)
Abstract
Isovitexin, isolated from rice hull of Oryza sativa, has been characterized as a potent antioxidant. Its antioxidant activity, determined on the basis of inhibition of lipid peroxidation by the Fenton reaction, was comparable with that of £\-tocopherol, a well-established antioxidant. Isovitexin was able to reduce the amount of hydrogen peroxide production induced by lipopolysaccharide (LPS) in mouse macrophage RAW264.7 cells. In this study, we assessed its effects on the production of tumor necrosis factor £\(TNF-£\), prostaglandin E2 (PGE 2 ), and the expression of cyclooxygenase-2 (COX-2) in LPS-activated RAW 264.7 macrophages. Isovitexin inhibited the release of TNF-£\, a proinflammatory cytokine, upon LPS activation with a 50% inhibitory concentration (IC 50 ) of 78.6 £gM. Isovitexin markedly reduced LPS-stimulated PGE 2 production in a concentration-dependent manner, with an IC 50 of 80.0 £gM. The expression of COX-2 was also inhibited by isovitexin treatment. Our results suggest that suppression of ROS-mediated COX-2 expression by isovitexin is beneficial in reducing inflammation and carcinogenesis. ? 2005 New York Academy of Sciences.
Subjects
Antioxidant
COX-2
Inflammation
Isovitexin
PGE 2
Tumor necrosis factor
Type
conference paper