Amaranthus spinosus L. Inhibits Spontaneous and Dexamethasone-Induced Apoptosis in Murine Primary Splenocytes
Resource
Journal of Food and Drug Analysis 16 (4): 52-61
Journal
Journal of Food and Drug Analysis
Journal Volume
16
Journal Issue
4
Pages
52-61
Date Issued
2008
Date
2008
Author(s)
Abstract
Amaranthus spinosus L. (thorny amaranth) is traditionally selected to be an anti-diabetic herb in Taiwan. This study assessed the immuno-modulatory effects of wild A. spinosus water extract (WASWE) on spontaneous and dexamethasone (DEX)-induced apoptosis in murine primary splenocytes. The cultured splenocytes treated with WASWE products were harvested and analyzed to assess their apoptotic status according to DNA fragmentation by flow cytometry and agarose gel electrophoresis. The results showed WASWE inhibited the spontaneous and DEX-induced apoptosis of splenocytes. Furthermore, both WASWE and its activated charcoal-treated sample administered at concentration of 1.250 mg/mL inhibited intracellular protein levels of p53 (an apoptotic inducer protein) and p80 (a form of tumor necrosis factor (TNF) receptor), which are involved in apoptosis. The present study suggested the existence of a heat-labile anti-apoptotic component with high molecular weight in WASWE.
Subjects
Anti-apoptosis; DNA fragmentation; Medicinal vegetables; Murine primary splenocytes; Wild Amaranthus spinosus water extract (WASWE)
SDGs
Other Subjects
Amaranthus spinosus extract; dexamethasone; plant extract; protein p53; protein p80; unclassified drug; agar gel electrophoresis; Amaranthus; amaranthus spinosus; animal cell; animal experiment; animal tissue; apoptosis; article; Bagg albino mouse; cell culture; controlled study; DNA fragmentation; drug activity; female; flow cytometry; immunomodulation; molecular weight; mouse; nonhuman; spleen cell; wild plant; Amaranthus caudatus; Amaranthus spinosus; Murinae
Type
journal article
File(s)![Thumbnail Image]()
Loading...
Name
28.pdf
Size
594.39 KB
Format
Adobe PDF
Checksum
(MD5):7952e0d82609eab28c2d3c50a3501d14