Ionic Conjugated Organic Molecules and Their Self-Assembled Nanospheres: Syntheses, Characterizations and Applications
Date Issued
2014
Date
2014
Author(s)
Lee, Kuan-Yi
Abstract
In this thesis, we designed a series of highly fluorescent π-conjugated organic molecules with ionic self-assembly motifs. Through the characterizations of electron microscope and dynamic light scattering, the molecules can spontaneously assemble into hollow vesicle-like nano-spheres with diameter of 100-400 nm in the acetone solutions. Moreover, we could systematically investigate the vesicular mechanism by manipulating the force balance of the self-assembly process in the way of changing solvent environments.
In addition, by utilizing the FRET mechanism, we could further probe the dynamic equilibrium of the self-assembly behavior and even molecular self-recognition ability in liquid phase. On the other hand, through the so-called non-solvent assisted separation method we could compel the blue-green nano-spheres with the same self-assembly behavior separating on a solid glass substrate, and therefore explored the inter-spherical energy transfer process in solid state. Finally, we compared the energy transfer efficiencies with the nano-systems that behave the self-sorting ability.
Because of the advantageous ionic nature and stability in the aqueous medium, we successfully internalized the nano-spheres into normal and cancer cells of lung tissues through the observation of confocal laser scanning microscope. The cell viability under different molecular concentrations was investigated by MTT assay. Moreover, we could obtain color tunable and even white-light nano-spheres for multi-channel imaging purposes by varying the composition of RGB components through intra-spherical energy transfer process attributed to the lack of molecular self-recognition. Finally, we utilized the flow cytometry to quantify the mean fluorescence intensity of nano-spheres within the living cells.
Subjects
自組裝
奈米球
細胞顯影
能量轉移
自分類
SDGs
Type
thesis
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