GM-CSF plays a key role in zymosan-stimulated human dendritic cells for activation of Th1 and Th17 cells
Resource
CYTOKINE, 55(1), 79-89
Journal
Cytokine
Pages
79-89
Date Issued
2011
Date
2011
Author(s)
Wei, Wen-Chi
Su, Yi-Hsuan
Chen, Swey-Shen
Sheu, Jyh-Horng
Yang, Ning-Sun
Abstract
In this study, we compared the effects of zymosan and LPS on human monocyte-derived dendritic cells. The specific effects of zymosan on the expression of several key cytokines, including granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukins (IL-1α, IL-1β and IL-12 p70) were quite distinct from the effects of LPS. Unlike activation with LPS, DCs activated by zymosan expressed little or no IL-12 p70 due to lack of expression of the p35 subunit. However, treatment with zymosan resulted in a substantial increase in Th1 and Th17 cell-polarizing capacity of DCs. Furthermore, the GM-CSF secreted by zymosan-activated DCs enhanced IL-23 production, resulting in activation of a Th17 response. GM-CSF and IL-27, rather than IL-12 p70, were both major direct contributors to the activation of a Th1 response. This signaling mechanism is distinct and yet complementary to LPS-mediated T-cell activation. We suggest that this novel zymosan-induced GM-CSF-mediated signaling network may play a key role in regulating specific immune cell type activities.
Type
journal article
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