The effect of growth factor on the induction of senescence-like phenotype of human dermal fibroblast
Date Issued
2011
Date
2011
Author(s)
Wang, Chan-Tia
Abstract
Introduction: The aging is a very important issue for human with the global trend of an aging population. Therefore, the youth of the key growth factor was become study object nowadays .Hence, the main objective was developing long-term effects of different growth factors for cell aging in this study.
Materials and methods: Human epidermal growth factor and fibroblast growth factor as a test of the growth factor. Primary skin dermal fibroblasts were cultured on two growth factor to establish senescence correlation index, such as growth curves and SA-β-galactosidase activity. Finally, the senescence associated gene analysis were carried out.
Results:Epidermal growth factor (EGF) was observed no significant difference from the P.D curve in the short-term period. In the long-term period, the P.D curve, cell morphology, Senescence-associated ß-galactosidase activity and aging-related gene expression were significantly similar to the aging of the type produced. In the experimental group of plus Fibroblast growth factor (bFGF), the P.D curves were observed after a long -term period that are significant differences in the number of cells representative of the ability to increase cell proliferation. And continuing the curve, the cell morphology, Senescence-associated ß-galactosidase activity and aging-related gene expression were significantly similar to the aging of the type produced.
Conclusion: According to the result of the sample containing Epidermal growth factor, Epidermal growth factor may not be able to enhance the growth of Fibroblast. In the Epidermal growth factor containing specimen, Epidermal growth factor may become a stress which induces the senescence and apoptosis of cell. In addition, the result of the sample containing Fibroblast growth factor shows increase of cell proliferation in early stage. (Generally, normal telomere has fixed length.) Fibroblast growth factor could increase the proliferation times of cell and therefore approach replicative senescence earlier than usual.
Subjects
Human fibroblasts
epidermal growth factor
fibroblast growth factor
cell aging
telomere theory
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-100-R98548034-1.pdf
Size
23.54 KB
Format
Adobe PDF
Checksum
(MD5):fc25584ccabcc552af9f1f6deb13ea10