Chitosan/Gelatin Hydrogel Prolonged the Function of Insulinoma/Agarose Microspheres In Vivo During Xenogenic Transplantation
Journal
Transplantation Proceedings
Journal Volume
40
Journal Issue
10
Pages
3623-3626
Date Issued
2008
Author(s)
Abstract
Purpose: A chitosan/gelatin solution with glycerol 2-phosphate disodium salt hydrate in liquid phase at room temperature becomes a hydrogel at 37°C. The material can be used as an injectable cell carrier into the human body for gelation in situ. We hoped that the chitosan/gelatin hydrogel provided extra protection for insulinoma/agarose microspheres during xenogenic transplantation. Materials and Methods: Mouse insulinoma was microencapsulated in agarose as microspheres, which were macroencapsulated in chitosan/gelatin hydrogel. Insulin secreting profiles were first demonstrated in vitro. Diabetic rats were injected subcutaneously with insulinoma/agarose microspheres or insulinoma/agarose microspheres suspended in chitosan/gelatin solution. The nonfasting blood glucose concentrations (NFBG) of diabetic rats were measured perioperatively. Rats were humanely killed 1 month postoperatively and the hydrogel was retrieved for histological examination. Results: The insulinoma/agarose microspheres continually secreted insulin for 1 month when macroencapsulated in chitosan/gelatin hydrogel in vitro. The NFBG of diabetic rats injected with insulinoma/agarose microspheres decreased to euglycemic status albeit hyperglycemia was restored within 10 days. The NFBG of diabetic rats injected with chitosan/gelatin hydrogel, which contained insulinoma/agarose microspheres, was maintained at less than 200 mg/dL for 25 days. The histological section revealed immune cell infiltration and accumulation within the hydrogel and around the iusulinoma/agarose microspheres that may have contributed to the slowly increasing NFBG after day 25. Conclusion: This study showed that chitosan/gelatin hydrogel can be used as a cell carrier for an injectable bioartificial pancreas; the hydrogel prolonged the function of cells encapsulated in agarose microspheres during xenogenic transplantation. ? 2008 Elsevier Inc. All rights reserved.
SDGs
Other Subjects
agarose; chitosan; gelatin; glucose; glycerol 2 phosphate; microsphere; animal cell; animal experiment; animal model; animal tissue; article; cell infiltration; concentration (parameters); diabetes mellitus; gelation; glucose blood level; hydrogel; hyperglycemia; immunocompetent cell; in vitro study; in vivo study; insulin release; insulinoma; liquid; microencapsulation; mouse; nonhuman; priority journal; protection; rat; room temperature; xenograft; xenotransplantation; Animals; Chitosan; Gelatin; Hydrogels; Insulin; Insulinoma; Mice; Neoplasm Transplantation; Rats; Sepharose; Transplantation, Heterologous
Type
journal article