Dissolved organic matter with multi-peak fluorophores in landfill leachate
Resource
CHEMOSPHERE,74(4),575-582.
Journal
CHEMOSPHERE
Journal Volume
74
Journal Issue
4
Pages
575-582
Date Issued
2008
Date
2008
Author(s)
Abstract
Dissolved organic matter (DOM) sampled from municipal landfill leachate of different ages with/without anoxic or aerobic treatment, was intensively fractionated via size exclusion chromatography (SEC) and hydrophobic resins, and was studied with fluorescence excitation and emission matrix (EEM). Six fluorophores with multiple EEM peaks (fluorophore A-F) were identified based on the collected EEM spectra and validated by bi-variate analysis, principal component analysis, and parallel factor analysis, as follows (excitation wavelength Ex and emission wavelength Em): (Ex 240, 310, 360 nm, Em 460 nm), (Ex 220, 280 nm, Em 340 nm), (Ex 220, 270 nm, Em 300 nm), (Ex 220, 280 nm, Em 360 nm), (Ex 230, 320 nm, Em 420 nm) and (Ex 220, 310 nm, Em 400 nm). The spectral characteristics of these fluorophores were discussed using fractional EEM and apparent molecular weight (AMW) data obtained via SEC analysis. The triple peak flurophore A was pointed at a hydrophobic acid or hydrophobic neutral compound with a pyrenyl functional group of AMW 2500-3500 Da, which displayed an excitation wavelength at 360 nm and a fluorescence intensity ratio of 6.70(+/-1.79):1.70(+/-0.41):1 (fluorescent intensities of Ex 240:Ex 310:Ex 360 nm at Ex 460 nm). This compound is observed to be refractory in landfilling or in anoxic/aerobic treatments, and is specific to this leachate contamination. This paper revealed that the coupling of SEC and EEM can be useful to track the fluorescent DOM fraction in landfill leachate.
SDGs
Other Subjects
Aerobic treatments; Apparent molecular weights; Dissolved organic matters; DOM fractions; Emission wavelengths; Excitation and emission matrix; Excitation wavelengths; Fluorescence excitations; Fluorescence intensity ratios; Fluorescent intensities; Flurophore; Hydrophobic acids; Hydrophobic neutrals; Hydrophobic resins; Landfill leachate; Leachate; Leachate contaminations; Municipal landfill leachate; Parallel factor analyses; Principal components; SEC analysis; Spectral characteristics; Biogeochemistry; Biological materials; Chromatographic analysis; Chromatography; Dissolution; Emission spectroscopy; Fluorescence; Fluorophores; Fractionation; Functional groups; Gel permeation chromatography; Hydrophobic chromatography; Hydrophobicity; Land fill; Leaching; Organic compounds; Organic polymers; Principal component analysis; Refuse disposal; Resins; Size exclusion chromatography; Waste disposal; Wastewater treatment; Leachate treatment; anoxic conditions; dissolved organic matter; fluorescence; hydrophobicity; landfill; leachate; oxic conditions; principal component analysis; resin; Chromatography, Gel; Fluorescent Dyes; Humic Substances; Molecular Weight; Organic Chemicals; Spectrometry, Fluorescence; Water Pollutants, Chemical
Type
journal article
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