Methyl Cellulose Enhance Gelatin Membrane as Guidance Channel for Periphral Nerve Regeneration
Date Issued
2008
Date
2008
Author(s)
Liu, De-Fu
Abstract
In the study, the dialdehyde cellulose-gelatin was designed and fabricated for peripheral nerve regeneration. The methyl cellulose could successfully transferred into 2, 3-dialdehyde cellulose by using sodium periodate as an oxidant. The DAC-Gel membrane with different conditions was fabricated by controlling the concentration of sodium periodate and the reaction time. The biological stability of membrane was improved by cross-linking with gelatin. The physical-chemical properties and biocompatibility of DAC-Gel membrane were evaluated to ensure the potential viability of this novel co-polymer for peripheral nerve regeneration.o estimate the properties of the DAC-Gel membrane with different conditions, the methyl cellulose was oxidized by different concentration of sodium periodate. After oxidized, the dialdehyde cellulose with different degree of oxidation was fabricated. FTIR could further confirm the formation of aldehyde group and the quantification of aldehyde group showed that the concentration sodium periodate was the dominant factor to the degree of oxidation. Basic assessment of cross-linking degree showed that steric hinderance could be the factor to hinder cross-linking. According to the degradation test, the DAC-Gel membrane could prolong the degradation time and slow down the rate of degradation. The hydrophilic/hydrophobic surface after cross-linking was evaluated by water contact angle test. The DAC-Gel membrane was more hydrophilic than the Gel membrane using glutaraldehyde as a cross-linker. The biocompatibility was evaluated by WST-1 cell proliferation and LDH cytotoxicity. The DAC-Gel membrane provided an adaptable place for cell proliferation and the membrane using glycine to block the residual aldehyde group showed a low cytotoxicity. To sum up, the DAC-Gel membrane was a newly designed material and possessed great potential in the different application.
Subjects
methyl cellulose
sodium periodate
2,3-dialdehyde cellulose
gelatin
peripheral nerve regeneration
Type
thesis
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