Transcription Factor Six1 Plays Function in Craniofacial Myogenesis of the Zebrafish
Date Issued
2007
Date
2007
Author(s)
Chen, Wei-Ta
DOI
zh-TW
Abstract
six1 gene belongs to the sine oculis/six gene family that plays important roles in morphogenesis, organogenesis, and cell differentiation. Studies in Drosophila have revealed that six family, eyeless (pax family), eyes absent (eya family) and dachshund (dach family) form a synergistic regulatory network to trigger compound eye organogenesis. six1 knockout mice showed that knockdown of six1 causes extensive muscle hypoplasia which affects most of the trunk muscles, indicating that six1 is required for trunk myogenesis. However, the function of six1 in craniofacial myogenesis remains unknown. To further understand the role of six1 in the development of head muscles, we used zebrafish as a model organism. In this study, I used whole mount in situ hybridization to observe the expression pattern of six1 in the head of zebrafish. Results showed that six1 was expressed in all head-muscle progenitor cells. Knockdown of six1 proved that Six1 was required for the development of sternohyoideus, medial rectus, inferior rectus, and all pharyngeal arch muscles. Six1 was also required for the expression of myod and myogenin in these muscles. Exogenous myod mRNA can rescue the loss of the cranial muscles caused by six1-morpholino(MO), indicating myod may be a target gene of Six1 to regulate craniofacial myogenesis. Moreover, we found that the defect of craniofacial muscles caused by pax3-MO phenocopied that of six1-MO. But exogenous six1, myod or myf5 mRNA did not rescue the loss of the cranial muscles caused by injecting pax3-MO, suggesting six1 and pax3 could not function in the same genetic network to regulate development of head muscles. In addition, we also observed that fin bud and posterior hypaxial muscles disappeared in six1 and pax3 morphants . Taken together, our results revealed that six1 played an important role in craniofacial myogenesis.
Subjects
頭部肌肉
craniofacial
Type
other
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