Highly reliable GIGA-sized synthetic human therapeutic antibody library construction
Journal
Frontiers in immunology
Journal Volume
14
Pages
1-12
Date Issued
2023-04-27
Author(s)
Huang, Chao-Yang
Lok, Ying-Yung
Lin, Chia-Hui
Lai, Szu-Liang
Wu, Yen-Yu
Hu, Chih-Yung
Liao, Chu-Bin
Ho, Chen-Hsuan
Chou, Yu-Ping
Hsu, Yi-Hsuan
Lo, Yu-Hsun
Abstract
Monoclonal antibodies (mAbs) and their derivatives are the fastest expanding category of pharmaceuticals. Efficient screening and generation of appropriate therapeutic human antibodies are important and urgent issues in the field of medicine. The successful in vitro biopanning method for antibody screening largely depends on the highly diverse, reliable and humanized CDR library. To rapidly obtain potent human antibodies, we designed and constructed a highly diverse synthetic human single-chain variable fragment (scFv) antibody library greater than a giga in size by phage display. Herein, the novel TIM-3-neutralizing antibodies with immunomodulatory functions derived from this library serve as an example to demonstrate the library's potential for biomedical applications.
Subjects
TIM-3; antibody therapeutics; phage display; single-chain variable fragment (scFv); synthetic antibody library
SDGs
Publisher
FRONTIERS MEDIA SA
Type
journal article