Analysis of functional domains in zebrafish Ugly duckling (Udu) protein
Date Issued
2014
Date
2014
Author(s)
Chang, Chia-Hua
Abstract
It has been shown that zebrafish Udu plays a role in many developmental processes. Our previous data showed that Udu has diverse functions, including erythrocyte development and may be involved in modulating DNA replication or cell cycle. Gon4l is one of the homolog of zebrafish Udu in human and mouse. During evolution, Gon4l was partially duplicated and produced a novel protein, YY1AP (YY1-associated protein). It has been demonstrated that when YY1AP binds to YY1, it will enhance the transcriptional activation of YY1-targeted promoter in human cells. Likewise, our previous data showed that YY1 can associate with YY1-binding domains (YY1BD) of Udu. According to these results, I hypothesize that Udu YY1BD, PAH and SANT domains might have respective or independent functions during development.
I used udu mutants, udutu24 and udusq3 to study whether N-terminal segment of Udu has distinct functions or not. Compared to udutu24, udusq3 remains one of YY1BD sequence in udu gene structure. By using hematopoietic and angiogenic markers for in situ hybridization, I found that there are different expression patterns in udutu24 and udusq3. Furthermore, I injected udu N-terminal or full-length mRNA into one-cell stage udutu24 or udusq3 embryos separately. I have shown that both udu N-terminal and full-length mRNA could partially rescue phenotypes in udu mutants. Moreover, I discovered that ISV defect in udusq3 is much more severe after yy1 MO injection. It suggests that YY1BD of Udu has distinct functions in zebrafish. To sum up, these data shows that YY1BD, PAH and SANT domains of Udu may play independent roles in different developmental processes.
Subjects
斑馬魚
血管新生
血管發育
血球發育
細胞凋亡
肌節間血管
Type
thesis
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