水管道蛋白於人體肺組織與細胞株之表現及調控
Other Title
Expression And Regulation of Water Channel Proteins In Human Lung
Tissues and Cell Lines
Tissues and Cell Lines
Date Issued
1999
Date
1999
Author(s)
郭炳宏
DOI
882314B002229
Abstract
Recent discovery of water channel
proteins (aquaporins, AQP) has provided
insight into the molecular mechanism of
water transport across the cell membrane.
Nevertheless, tissue distribution and the
pathophysiology of these proteins in lung
disorders remain unclear. This study
applied techniques in molecular biology to
screen human lung cell lines for the
expression of known aquaporin genes, and
also investigated tissue distribution of
aquaporins in adult human lung and pleura
by immunohistochemistry. Lung tissue
sections, obtained during lobectomy, were
analyzed by immunohistochemistry and
immunoblot with aquaporin antibodies.
Expression of AQP3 mRNA and protein is
identified in A549 cells. No other cell
lines of human airway epithelium and
pleura in our laboratory were positive for
other AQP genes. AQP3 mRNA
expression in the dexamethasone-treated
group (10 -5 M for 8 h) increased to
approximately five times the level of that
in the control group but did not change
significantly after incubation with IL-2 (1 nM) for 8 hrs.
Immunohistochemistry and Western
blotting revealed that AQP1 is expressed
in all portions of the lung, AQP3 is less
strongly expressed in trachea and not
detectable in distal lung. AQP5 is
expressed in distal lung. Data obtained
from his study might provide the basis for
future study regarding the regulation and
pathophysiology of aquaporins in human
lung water homeostasis.
Subjects
水管道蛋白
人體肺組織與細胞株
基因表現及調控
Aquaporin
Water channels
Human lung tissues and cell line
Gene
expression
expression
Tissue distribution
Publisher
臺北市：國立臺灣大學醫學院內科
Type
report
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