The expression of porcine adiponectin and stearoyl coenzyme a desaturase genes in differentiating adipocytes
Journal
Asian-Australasian Journal of Animal Sciences
Journal Volume
17
Journal Issue
5
Pages
588-593
Date Issued
2004
Author(s)
Abstract
For cloning of gene fragments and tissue distribution studies, three crossbred pigs (Sus domesticus; sows were predominantly Landrace-Yorkshire crossbreds mated to a Duroc boar) at age 8 to 9 wk old were killed, with an electrocution combined with exsanguinations 2 h after feeding. The protocol was approved by the Experimental Animal Management and Use Committee at National Taiwan University. Heart, kidney cortex, liver, longissimus muscle, and subcutaneous adipose tissue were quickly dissected and frozen in liquid nitrogen with storage at -70C until RNA extraction.
Type
journal article