發酵糖類釋出氣體的兼氣性海洋細菌之研究﹝二﹞
Date Issued
2000
Date
2000
Author(s)
DOI
892313B002086
Abstract
A search for halophilic, facultatively
anaerobic bacteria that ferment carbohydrates
with gas production has been made in the
present study. The most-probable-number
( MPN ) counting values of these bacteria in the
coastal seawater collected from northern
Taiwan were estimated to be in the range of 101
to 104 cells/100 ml. Forty-three bacterial strains
that ferment carbohydrates with gas production
were isolated from the MPN counting tubes.
3
Twelve lost the gas-producing property after
subculture among them. All the other isolates
were Gram-negative, and motile by polar
flagella. They were able to grow in a mineral
medium containing D-glucose, and could
ferment carbohydrates with gas production.
These isolates required Na+ for growth.
According to phenotypes, thirty-one strains
were divided into five groups. Group 1
contained only one strain, which produced red
colonies. The cells were curved rods in shape
and oxidase-negative. The strain was able to
assimilate mannitol as sole carbon source, and
reduced nitrate. Group 2 contained six strains,
which produced white and transparent colonies.
The cells were curved rods in shape and
oxidase-negative. The strains were able to
assimilate mannitol as sole carbon source, and
reduced nitrate. Group 3 contained three strains,
which also produced white and transparent
colonies. The cells were curved rods in shape
and oxidase-positive. The strains were able to
assimilate mannitol as sole carbon source, and
couldn’t reduce nitrate. Group 4 contained
three strains, which produced white and opaque
colonies. The cells were short and straight rods
in shape and oxidase-positive. The strains
weren’t able to assimilate mannitol as sole
carbon source, and reduced nitrate. Group 5
contained eighteen strains, which produced
white and transparent colonies. The cells were
curved rods in shape and oxidase-positive. The
strains were able to assimilate mannitol as sole
carbon source, and reduced nitrate. The levels
of 16S rDNA sequence similarity compared
with any sequences available in the GeneBank
database revealed that the representative strains
NFG1 and NFG3 of Group 1 and Group 3
could be considered to represent various new
species. The representative strain NFG4 of
Group 4 was identified as Photobacterium
damselae subsp . damselae. The taxonomic
status of NFG2 and NFG5 that representative
strains of Group 2 and Group 5 couldn’t be
determined.
Subjects
halophilic
ferment carbohydrates with gas production
16S rDNA sequence
Publisher
臺北市：國立臺灣大學海洋研究所
Type
report
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