Vacuum pouch microfluidic system for the application of digital PCR
Journal
MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Pages
272-273
Date Issued
2020
Author(s)
Abstract
In this paper, we present a digital PCR chip based on the vacuum pouch microfluidic (VPM) system. The uniqueness of this system is: Its thin-film structure can achieve a rapid thermal cycling process; It has a build-in vacuum pouch to generate digital droplet array on-chip. The total distance from the heater to the chamber is 30 μm, and a total of 35 thermal cycles can be completed in 26.5 minutes. To verify the feasibility of this system, miRNA-15b at a range of 125 to 1000 cps/μl are studies. The positive-to-negative ratio of fluorescent intensity of droplets is 10.37, and concentrations of 250 copies and 1000 copies of miRNA-15b can be digitally quantified. Experimental studies also demonstrated that 1000 droplets array can be generated by using the liquid-oil two-phase system. ? 2020 CBMS-0001
Subjects
Drop formation; RNA; Thermal cycling; Digital pcr; Droplet arrays; Fluorescent intensity; Micro fluidic system; Rapid thermal cycling; Thin-film structure; Total distances; Two phase systems; Digital microfluidics
Type
conference paper