The intranasal delivery study of liposomal-vaccine to SPF chickens
Date Issued
2009
Date
2009
Author(s)
Tseng, Li-Ping
Abstract
Liposomal-NDV vaccine intranasal administration to chicken can induce specific-NDV antibody at mucosal and serum. The adjuvant effect of multi-lamellar vehicles (MLVs) liposomes formulated with three phospholipids including phosphatidylcholine-liposomes (PC-Lip), phosphatidylserine-liposomes (PS-Lip), and stearylamine-liposomes (SA-Lip) was compared with that for virus alone using the inactivated Newcastle disease virus (NDV) as model antigen. PC-Lip and PS-Lip induced more NDV antibody titer after secondary immunizations. In response to virulent viral challenge, all control animals died, whereas 80~90% of animals which still survived. Added LPS with liposomal-NDV vaccine which enhanced chickens produced antibody in serum and nasal/tracheal lavages. Furthermore, we combined tremella with liposomal-NDV vaccine to enhance the muco-adhesive property and prolong liposomes on the mucosal surface. Chickens received T-PC-Lip resulted in a significant increase in HI titer also elicited a significant mucosal secretary immunoglobulin A (s-IgA) response in tracheal lavages and a serum IgG response. After virulent virus challenge, the protection rate of T-PC-Lip vaccine showed 100% survival rate. The molecular mechanism of liposomal-adjuvant was study both in vitro and in vivo. Confocal laser scan microscopy showed that PC-Lip were uptaken into chicken spleen macrophages. PC-Lip and PS-Lip stimulated chickens spleen macrophages produce NO. In response to PC-Lip stimulated, Bay 11-7085 and U0126 inhibited macrophages produced NO. PC-Lip was shown to be involved in both activation of NF-κB and upstream ERK of MEK pathway. Moreover, PC-Lip and T-PC-Lip were shown to be activated ERK pathway in vovo. In conclusion, T-PC-Lip is an active adjuvant for chickens resulting in trigger Th1 pathway of cellular immunity and Th2 pathway of humoral immunity.
Subjects
liposome
adjuvant
mucosal
NDV
nitric oxide
mucoadhesive
Tremella
SDGs
Type
thesis
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