Investigation of signal transduction pathway involved in plasma membrane repair of epithelial cells
Date Issued
2008
Date
2008
Author(s)
Tsai, Ping-Tsun
Abstract
Plasma membrane disruption is a common type of cell injury for cells enduring physiologically generated mechanical forces, such as epithelia and skeletal muscle cells.epair of torn cell surface is essential to cell survival and has been proposed to be mediated by calcium-elicited gel barrier formation and exocytosis. However, the exact mechanisms underlying membrane repair remain still obscure for years owing to the lack of methods to induce large-scale mechanical rupture of cell membrane in vitro.erein we characterize a “magnetic beads rolling” technique modified from the beadloadingethod (McNeil and Warder 1987) for quantitative analyses. Pilot experimentsuggested the involvement of CaMK in this process and protein levels of somenteresting targets were markedly changed during resealing process including severalR stress-related molecules. Importantly, we found that p38 MAPK and its upstreamEK3/6 and ASK-1 were transiently activated and inhibition of p38 impairedmembrane repair. We also observed compensatory endocytosis after membrane rupture.hese results suggest that membrane disruptions employ similar mechanism as othertress responses through p38 MAPK pathway. Equipped with this newly developedssay, we are in the processes of dissecting the signal transduction pathway involved inembrane rupture and repair.
Subjects
Membrane repair
stress response
beads rolling
exocytosis
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