Detection of rat parvovirus type 1 and rat minute virus type 1 by polymerase chain reaction
Resource
Laboratory Animals,40(1),63-69.
Journal
Laboratory Animals
Journal Volume
40
Journal Issue
1
Pages
63-69
Date Issued
2006-01
Date
2006-01
Author(s)
Abstract
Two newly recognized parvovirus species, rat parvovirus 1 (RPV-1) and rat minute virus 1 (RMV-1), were recently identified in naturally infected rats. In this study, two polymerase chain reaction (PCR) assays were developed to specifically detect RPV-1 and RMV-1. The RPV-1 PCR assay amplified the expected 487-bp deoxyribonucleic acid (DNA) fragment only in the presence of RPV-1 DNA; the RMV-1 PCR assay amplified the expected 843-bp product only from RMV-1 DNA, not from other rodent parvoviruses. The RPV-1 and the RMV-1 PCR assays detected approximately 18 and 70 copies of DNA template, respectively. These two PCR assays were shown to be sensitive, specific and rapid methods for detecting RPV-1 and RMV-1 infections in rats. These assays may also be valuable for evaluation of biological specimens for parvovirus contamination. ? Laboratory Animals Ltd.
SDGs
Other Subjects
virus DNA; animal tissue; article; controlled study; diagnostic test; diagnostic value; DNA extraction; DNA sequence; DNA template; evaluation; gene amplification; nonhuman; Parvovirus; polymerase chain reaction; rat; rat minute virus 1; rat parvovirus 1; sensitivity and specificity; viral contamination; virus detection; virus gene; virus infection; virus morphology; Animals; Animals, Newborn; Biological Assay; Cricetinae; DNA Primers; DNA, Viral; Humans; Mice; Minute virus of mice; Parvoviridae Infections; Parvovirus; Polymerase Chain Reaction; Rats; Rodent Diseases; Sequence Analysis, DNA; Animalia; DNA viruses; Murinae; Parvovirus; Reindeer papillomavirus; rodent parvoviruses
Type
journal article