Plasma proteome profiling of von Hippel-Lindau disease after total and subtotal nephrectomy: A preliminary study
Resource
CLINICAL BIOCHEMISTRY, 43(1-2), 142-149
Journal
Clinical Biochemistry
Pages
142-149
Date Issued
2010
Date
2010
Author(s)
Opatrna, Sylvie
Chiangjong, Wararat
Korabecna, Marie
Sintiprungrat, Kitisak
Sinchaikul, Supachok
Chen, Shui-Tein
Matejovic, Martin
Thongboonkerd, Visith
Abstract
Objectives: Common treatment of renal cell carcinoma associated with von Hippel-Lindau (VHL) disease is total (bilateral) or subtotal nephrectomy. Whereas total nephrectomy is associated with absolutely no residual renal function, subtotal nephrectomy frequently leads to chronic kidney disease (CKD) with some residual renal functions. However, molecular mechanisms underlying CKD remain unclear and the diagnosis of CKD is frequently accomplished at its late stage. Design and methods: We performed a plasma proteomics study to compare the plasma proteome profile of VHL patient who underwent total nephrectomy to the profiles of VHL patient with subtotal nephrectomy and healthy control. Totally 100?μg proteins from each sample was resolved by two-dimensional electrophoresis (2-DE) in triplicate and visualized with SYPRO Ruby fluorescence stain. Results: The normal plasma proteome profile markedly differed from the profiles of VHL patients. Comparative analysis between total versus subtotal nephrectomized patients revealed significant differences in levels of 20 plasma proteins. Pathway analysis revealed two important networks involving in lipid metabolism, molecular transport, carbohydrate metabolism, cellular growth and proliferation, and small molecule biochemistry, in which these identified and other proteins interplayed. Conclusions: Our data identified potential biomarkers for CKD. Further characterization of these identified proteins might also lead to better understanding of molecular mechanisms underlying CKD. ? 2009 The Canadian Society of Clinical Chemists.
Subjects
Chronic kidney disease; Nephrectomy; Plasma; Proteome; Proteomics; Renal cell carcinoma; Renal failure; von Hippel-Lindau
SDGs
Other Subjects
proteome; adult; aged; article; biochemistry; carbohydrate metabolism; case report; cell growth; cell proliferation; chronic kidney disease; comparative study; controlled study; female; fluorescence; human; kidney carcinoma; kidney function; lipid metabolism; male; matrix assisted laser desorption ionization time of flight mass spectrometry; nephrectomy; nucleotide sequence; pathogenesis; pathophysiology; priority journal; protein analysis; protein blood level; proteomics; two dimensional electrophoresis; von Hippel Lindau disease; Adult; Aged; Blood Proteins; Carcinoma, Renal Cell; Electrophoresis, Gel, Two-Dimensional; Female; Humans; Kidney Failure, Chronic; Kidney Neoplasms; Male; Nephrectomy; Organometallic Compounds; Proteome; Tumor Markers, Biological; von Hippel-Lindau Disease
Type
journal article
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