KIT gene exon 11 mutations in canine malignant melanoma
Journal
Veterinary Journal
Journal Volume
196
Journal Issue
2
Pages
226-230
Date Issued
2013
Author(s)
Abstract
The proto-oncogene KIT encodes a receptor tyrosine kinase which has been shown to be upregulated in canine melanomas. KIT mutations lead to constitutive phosphorylation and activation of KIT in the absence of ligand binding. The presence of KIT mutations and KIT protein expression was examined in a cohort of 49 dogs with canine malignant melanoma. An exon 11 synonymous nucleotide 1743C. → T mutation was identified in five cases in which one also harbored a L579P mutation. Tumors that harbored the KIT exon 11 mutation(s) correlated significantly with disease recurrence (P= 0.05). All 36 melanomas available for immunohistochemical analysis showed either weak (16 cases, 44.4%) or strong (20 cases, 55.6%) expression of the KIT protein. The five KIT mutation carriers were all strongly positive for KIT by immunohistochemical staining. These findings suggest that a subset of canine malignant melanomas harbors a KIT exon 11 mutation. ? 2012 Elsevier Ltd.
Subjects
Dog; Immunohistochemistry; KIT; Melanoma; Mutation
SDGs
Other Subjects
nucleotide; oncoprotein; protein KIT; unclassified drug; animal tissue; article; cancer recurrence; controlled study; dog disease; exon; female; gene mutation; heterozygote; immunohistochemistry; KIT gene; male; melanoma; nonhuman; nucleic acid analysis; protein expression; proto oncogene; Animals; Dog Diseases; Dogs; Exons; Female; Gene Expression Regulation, Neoplastic; Male; Melanoma; Mutation; Proto-Oncogene Proteins c-kit; Soft Tissue Neoplasms; Canis familiaris
Type
journal article