Effect of Temperature and Nutrient on the Expression of Rice Sucrose Transporter Genes
Date Issued
2012
Date
2012
Author(s)
Chung, Ping
Abstract
Sucrose is the major carbohydrate for long-distance transport in higher plants. Sucrose transporter (SUT) plays an important role to load sucrose into phloem for further transport, and it is also in charge of sucrose unloading from phloem. Rice SUT gene family are composed of five members (i.e. OsSUT1, OsSUT2, OsSUT3, OsSUT4 and OsSUT5). This study was focused on temperature and nitrogen nutrient effects on OsSUT gene expressions at seed germination, seedling growth and pollen maturation stages. The result showed that expressions of OsSUT1, 2 and 4 in embryos of germinating seeds at 30℃ and 37℃ were higher than that at 20℃. Since the effects of temperature on OsSUT1, 2 and 4 expressions in isolated embryos were different with that in embryos of whole seeds, it was suggested that endosperms might provide some factors such as sugars to regulate OsSUT gene expressions in embryos. In addition, the promoter analysis data showed that the OsSUT4 promoter activity responsed to different temperatures were consistent to the gene expression patterns. During pollen maturation, the expressions of OsSUT1, 3 and 4 in anthers were lower at 15/13℃ compared with that at 30/25℃ and 35/30℃. In the study related to nutrient effect on OsSUT expressions, it was indicated nitrogen starvation would enhance OsSUT1, 2 and 4 expressions in shoots. On the other hand, ammonium function as a positive factor to regulate OsSUT1 expression in root tissues.
Subjects
sucrose transporter
rice(Oryza sativa L.)
temperature
ammonium nitrogen
nitrogen starvation
Type
thesis
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