A Study on an Integrated Miniature Piezoresistive icrocantilever Biosensor and its Applications
Date Issued
2009
Date
2009
Author(s)
Yen, Yi-Kuang
Abstract
Diagnosis of complex diseases requires a quantitative and reliable detection of target proteins and pathogens. A portable, low-cost, and label-free biosensing tool is needed for diagnosis which is performed at the place far away from the examining laboratory or in the remote region. This work has developed an integrated miniaturized piezoresistive microcantilever-based biosensing platform for detections of the disease relevant protein and the pathogen. The sensing microcantilever was made up of multi-layer thin films of 1.2 μm in total thickness, of 300 μm in length and of 50 μm in width. The deflection sensitivity of the biosensing cantilever is experimentally measured to be , and the minimum detectable surface stress achieves 0.065 N/m. A disposable package was the first time being developed by integrating a sensor chip, a microfluidic device, and a platinum temperature sensor on a PCB for the convenience of a portable biosensing platform. single free-standing self-sensing piezoresistive microcantilever connected with a Wheatstone bridge electrical readout was proposed to avoid the unwanted false signals caused by the response of a reference cantilever to chemical effects during biochemical detections. Temperature effects as the noise sources also have been reduced by the temperature control system and the thermal-effect elimination method. oreover, this work also presented that the integrated miniature piezoresistive microcantilever has successfully been used to detect a clinically relevant biomarker, CRP, and a cultured fish pathogen, P. piscicida in a good reproducibility. For the assay of CRP, the analytical sensitivity achieves to diagnostic level of 1 μg/ml, and the CV % ranges from 17 % to 21%. In addition, for the preliminary study on the detection of P. piscicida, the CV% is found to be below 5%. The specificity of both these detections has been verified by processing control experiments. These biosensing applications demonstrated the reproducibility of this portable integrated biosensor which expresses its feasibility of commercialization.
Subjects
Biosensor
Piezoresistive microcantilever
MEMS
Protein detection
Pathogen detection
Type
thesis
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