DNA grouping, Serotyping, Pathogenicity, and Serological Survey of the Fowl Adenovirus Isolated in Taiwan
Date Issued
2004
Date
2004
Author(s)
Chang, Hsou-Mei
DOI
zh-TW
Abstract
Eight fowl adenoviruses (FAVs) were isolated from day-old chicks without clinical signs. The DNA of these FAVs was extracted and analyzed by the restriction enzyme digestion method as described by Zsak and Kisary (Intervirology 22:110-114, 1984). The result indicated that FAV strains isolated in Taiwan can be grouped into two major DNA groups, A and C. Further comparison based on the method by Raue and Hess (J Virol Methods 73:211-217, 1998), showed that the eight Taiwanese FAVs were assigned into the same two DNA groups and corresponded to the serotype 1 and 4 (FAV-1&FAV-4). The virus neutralization test of the eight FAVs also showed the same result. The virulence of Taiwanese isolates was studied in day-old specific-pathogen-free (SPF) chicks. Each chick was orally inoculated with one strain of the virus at the dose of 103 TCID50. The results revealed that two DNA-group A strains significantly retarded the growth of the chicks with immunosuppression but the DNA-group C trains did without immuno- suppression. Serums from the flocks that the eight Taiwanese strains were isolated from were tested for virus-neutralizing antibody against 10 serotypes of FAV. In addition to FAV-3 in flock B, the antibody positive rates of the other serotypes were between 4.2% and 91.7%. The virus-neutralizing test was also used to examine serum obtained from white broilers, hybrid broilers, and breeder chickens in Taiwan. The seroprevalence of FAV-1, FAV-4, and FAV-8 were 58.1%, 12.9%, and 70.4% respectively in white broilers younger than 2 weeks, and 46.4%, 12.1%, and 69.0% respectively in breeders older than 25 weeks. The other age flocks showed very low antibody positive rates of FAV-1 and FAV-4, but the antibody positive rate of FAV-8 in hybrid broilers older than 8 weeks was higher than 76.0%. The result revealed that FAV-8 infection was the most common of them in all flocks. Antibodies against FAV-1 and FAV-8 were not detected in serum samples from the 1080 and 888 pigeons collected between Oct. 2000 and Sep. 2001 in Taiwan.
Subjects
基因群
雞
家禽腺病毒
血清型
血清學調查
病原性
DNA groups
chicken
fowl adenovirus
serotype
serological survey
pathogenicity
Type
thesis
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