Characterization of the mouse LCN2 receptors in macrophage
Date Issued
2011
Date
2011
Author(s)
Wang, Mu-Yi
Abstract
Abstract
LCN2 belongs to the lipocalin family and performs as an acute phase protein. It can be internalized into the cells via receptor-mediated endocytosis and play a role as an iron transporter. To our knowledge, two LCN2 membrane receptors, 24p3R and megalin, may be in charge of the LCN2 response. According to our previous studies, the level of LCN2 was highly induced in uterus and peritoneal fluid during the development of endometriosis. The administration of LCN2 antibody would diminish the ectopic endometriosis formation. It indicates the significance of LCN2 in reproductive system. LCN2, as an iron-transporter，may activate the macrophage in peritoneal cavity and trigger the secretion of cytokines from macrophage during the endometriosis formation via iron-internalization by LCN2. Currently，we attempt to confirm whether the macrophage can internalize the iron via LCN2 receptor-mediated endocytosis. Furthermore, using shRNA analysis, we identified the real receptor of LCN2 endocytosis in macrophage. The results showed that the iron could be transported the iron into the cell via LCN2-initicated endocytosis, and suggesting the 24p3R is an essential mediator. After establishing a mouse endometriosis model, we examined the gene expressions of LCN2、24p3R and megalin in the possibilities of endometriosis development. The results demonstrated that LCN2 internalized into the macrophage via 24p3R and might be associated with the formation of endometriosis. Megalin seemed to do nothing with LCN2 in macrophage endocytosis and endometriosis formation.
Subjects
endometriosis
macrophage
acute phase protein
Type
thesis
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