Application of high-performance liquid chromatography-solid phase extraction-nuclear magnetic resonance in the identification of bioactive constituents from Hyptis spicigera leaf and H. brevipes aerial part
Journal
Journal of the Chinese Chemical Society
Series/Report No.
Journal of the Chinese Chemical Society
Journal Volume
71
Journal Issue
6
Start Page
619
End Page
629
ISSN
0009-4536
Date Issued
2024-06-01
Author(s)
DOI
10.1002/jccs.202400029
Abstract
AbstractTwo Hyptis plants endemic to Taiwan, H. rhomboides and H. suaveolens, contained xanthine oxidase inhibitors. To chemically investigate more herbs of the same genus, this study aimed to explore bioactive constituents from Hyptis spicigera leaf and the aerial part of H. brevipes, which would also enrich the chemotaxonomic basis for the Hyptis plants. The EtOH extract of the plant materials was fractionated by liquid‐liquid partitioning, followed by separation over various chromatography. High‐performance liquid chromatography‐solid phase extraction‐nuclear magnetic resonance hyphenation, a sustainable and environmentally friendly instrument, was applied to accelerate the chemical investigation of each subfraction. Eighteen isolates from Hyptis spicigera leaf and 14 compounds from H. brevipes aerial part were characterized. Of these, 12 compounds are rosmarinic acid derivatives, of which 9′‐O‐methyl melitrate A (10) and sagecoumarin methyl ester (22) are tentatively identified as new compounds. In total, 25 compounds were separated and characterized from Hyptis spicigera leaf and H. brevipes aerial part. Of these, seven of them (2–4, 11, 12, 18, 20) are in common between these two species, and 17 isolates (2–4, 8‐14, 22, 23, and 26‐30) are phenylpropanoids which are commonly distributed in the Labiatae family.
SDGs
Publisher
Wiley
Type
journal article