The taxonomy and phylogenetic analyses of microsporidian species isolated from Ocinara lida Moore (Lepidoptera: Bombycidae)
Date Issued
2008
Date
2008
Author(s)
Wang, Chih-Yuan
Abstract
A new microsporidium, Vairimorpha ocinarae n. sp., was isolated from the moribund larvae of Ocinara lida Moore (Lepidoptera: Bombycidae), a pest of Ficus spp. in Taiwan. This isolate causes a systemic infection of O. lida larvae. The midgut epithelium, midgut muscle tissues, and Malpighian tubules were the main target tissues for this isolate, and the atrophied fatty body was found in the seriously infected larvae. Two types of spores were found: binuclear spores possessed 11 to 13 polar-tube coils; mononuclear spores possessed 12 coils polar tube and aggregated usually to form octospores. Electron-dense episporontal inclusions filled in the episporontal space, and this structure was similar to that of Vairimorpha sp. NIS-M11 isolated from Bombyx mori and V. invictae isolated from Solenopsis invicta, but different from other Vairimorpha species. Based on the phylogenetic analysis of SSUrDNA sequences, this isolate is closely related to Vairimorpha sp. NIS-M11 and Vairimorpha sp. NIK-3h. Morphological and genetic characters showed that this isolate is a new species placed in the genus Vairimorpha. The species and isolates in the Vairimorpha complex were analyzed phylogenetically based on the sequences of rDNA (SSUrDNA and LSUrDNA), ITS (HG4F-HG4R region, ITS+580R region, and 580R region), protein coding genes (alpha-tubulin, beta-tubulin and hsp70 genes), and total sequenced genes. The four groups were identified and the relationships of inter-groups and intra-groups were evaluated. Based on phylogenetic analyses of total sequence genes and RFLP-PCR of SSUrDNA, the Group 3 and 4 are closer to Group 2 than Group 1. The results of the phylogenetic analyses based on the sequences of each examined gene or region were evaluated for the group identification. The internal transcribed spacer (ITS) is the best candidate for identification of inter-groups and intra-groups. The second candidate is protein coding genes. In the Endoreticulatus complex, there were two groups formed based on the phylogenetic analyses of SSUrDNA. The identities of inter-groups and intra-groups were both 98~99%. It still needs more gene sequence evidences to confirm the relationships between intra-groups and inter-groups.
Subjects
Vairimorpha ocinarae n. sp.
Ocinara lida
phylogenetic analysis
ultrastructure
electron-dense granule
Endoreticulatus complex
Vairimorpha complex
Type
thesis
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