Dissection of genetic factors controlling tomato stamen length around se2.1 QTL using substitution mapping and association mapping
Date Issued
2010
Date
2010
Author(s)
Liu, Chu-Yin
Abstract
The degree of stigma exserted above the stamen is a key determinant of mating behavior in tomato. Se2.1 was previously identified as a quantitative trait locus controlling stigma exsertion and is a gene complex affecting style length, stamen length, and anther dehiscence. In the current study, genetic components of se2.1 were further investigated using association mapping and substitution mapping.
For association mapping, one hundred Solanum lycopersicum var.cerasiforme accessions and ninety nine S. pimpinellifolium accessions from TGRC were used. Sixty three randomly chosen CAPS markers from the whole tomato genome were used to estimate population structure and defined two sub-populations for the S. pimpinellifolium accessions analyzed. Twenty nine CAPS markers within a 214.78kb genomic contig which contains Style2.1, Stamen2.2, and Stamen2.3 were genotyped to estimate the range of linkage decay in S. lycopersicum var cerasiforme and S. pimpinellifolium populations, respectively. Only the S. pimpinellifolium population showed very short linkage decay and was suitable for high-resolution genetic mapping. The result of association mapping delineated the genetic variations of Style2.1 attributed to phenotypic variations of style length at two sites: one close to the start codon and the other close to the 450bp indel located at 4kb upstream of the start codon. In addition, a statistically significant association between marker TAP109 and stamen length was detected within the genomic region containing Stamen2.2 and Stamen2.3.
For substitution mapping, nearly isogenic lines delimiting genes responsible for stamen length in the previous study were evaluated again. In additional to Stamen2.1, existence of additional gene controlling stamen length was suggested.
Subjects
association mapping
substitution mapping
stamen
tomato
Type
thesis
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