Interaction of viral RNAs derived from infectious clones of Papaya leaf distortion mosaic virus (PLDMV) and Papaya ringspot virus-Deformation strain (PRSV-DF) in different papaya cultivars
Date Issued
2015
Date
2015
Author(s)
Yao, Shun-Min
Abstract
Papaya ring spot disease caused by Papaya ringspot virus (PRSV) is a main limiting factor for the papaya industry in Taiwan. Papaya leaf distortion mosaic virus (PLDMV) is a newly emerged virus of papaya. The pathological characteristics of PLDMV such as symptoms, transmission and ecological niche are similar to those of PRSV-deformation strain (PRSV-DF). Therefore, PLDMV is considered to be a potential threat for papaya industry. This study is dedicated to investigate the pathological differences in different papaya cultivars and the interaction in the hosts between PLDMV and PRSV-DF by the transfection using artificial viral RNA transcripts generated in vitro from the PLDMV and PRSV-DF infectious clones constructed in lab previously. The simultaneous and asynchronous inoculation tests in different papaya cultivars are conducted in this study to understand various statuses of co-infection. The results of area under disease progress curve (AUDPC) and dynamic of virus multiplication monitored by the real-time RT-PCR assays will be used to evaluate the interaction between PLDMV and PRSV-DF. Although the symptoms of the TN2 papaya could not be made a distinction between simultaneous infection and single infection of PLDMV or PRSV-DF, the results demonstrated that the symptom progression of PLDMV single inoculation and asynchronous inoculation (PLDMV previous) was slightly slower than other treatments. The symptom severity of PRSV-DF single infected RL papaya was the mildest one among all treatments. Furthermore, there were no significant differences between PLDMV single infection and mix infection in the RL papaya. Both in the TN2 and RL papaya, the amount of PLDMV and PRSV-DF was 107 to 108 copy numbers and PRSV-DF was more than PLDMV generally. The asynchronous tests presented that the later invasive virus did not affect the replication of previous virus. These findings suggest that there is no synergistic interaction of PLDMV with PRSV-DF. It seemed that these two viruses could exist independently in the field and PRSV-DF was more predominant. PLDMV may take big challenges when only focusing on the resistance to PRSV-DF in future.
Subjects
Papaya ringspot virus-deformation strain
Papaya leaf distortion mosaic virus
infectious clone
interaction
Type
thesis
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