Determination of Gold Ions in Human Urine Using Genetically Engineered Microorganisms on a Paper Device
Journal
ACS Sensors
Date Issued
2018-03
Author(s)
Abstract
This paper presents a whole-cell biosensor that operates in conjunction with a smartphone-based fluorescence diagnostic system on a paper device to monitor the concentration of gold ions in human urine. The heavy metal-tolerant bacteria Cupriavidus metallidurans was genetically engineered for use as a chassis in a red fluorescent protein (RFP)-based microbial sensor. The biosensor is highly sensitive to gold ions, with a detection limit of 110 nM. The proposed smartphone-based analysis system provides a user-friendly approach to design tools of personal health monitoring for reporting the presence of gold ions in human urine. ? 2018 American Chemical Society.
Subjects
cueR; Cupriavidus metallidurans; paper device; smartphone-based diagnostic system; whole-cell biosensor
SDGs
Other Subjects
gold; ion; luminescent agent; photoprotein; red fluorescent protein; chemistry; Cupriavidus; genetic engineering; genetic procedures; genetics; human; paper; procedures; urine; Biosensing Techniques; Cupriavidus; Genetic Engineering; Gold; Humans; Ions; Luminescent Agents; Luminescent Proteins; Paper
Type
journal article