Anti-proliferation effect on human breast cancer cells via inhibition of pRb phosphorylation by Taiwanin E isolated from Eleutherococcus trifoliatus
Journal
Natural Product Communications
Journal Volume
9
Journal Issue
9
Pages
1303-1306
Date Issued
2014
Author(s)
Abstract
Eleutherococcus trifoliatus has been used as a folk medicine since ancient times, especially as refreshing qi medicines. In our current study, taiwanin E, which possesses strong cytotoxicity, was isolated from the branches of E. trifoliatus by using a bioactivity guided fractionation procedure. Taiwanin E presented a potent anti-proliferation activity on the growth of a human breast adenocarcinoma cell line (MCF-7), with an IC50value for cytotoxicity of 1.47 μM. Cell cycle analysis revealed that the proportion of cells in the G0/G1phase increased in a dose-dependent manner (from 79.4% to 90.2%) after 48 h exposure to taiwanin E at a dosage range from 0.5 to 4μM. After treatment with taiwanin E, phosphorylation of retinoblastoma protein (pRb) in MCF-7 cells was inhibited, accompanied by a decrease in the levels of cyclin D1, cyclin D3and cyclin-dependent kinase 4 (cdk4) and cdk6; in addition, there was an increase in the expression of cyclin-dependent kinase inhibitors p21WAF-1/Cip1and p27Kip1. The results suggest that taiwanin E inhibits cell cycle progression of MCF-7 at the G0/G1transition. ? 2014, Natural Product Incorporation. All rights reserved.
Subjects
Araliaceae; Cell cycle; Cytotoxicity; Eleutherococcus trifoliatus; G1arrest; Taiwanin E
SDGs
Other Subjects
cyclin D1; cyclin D3; cyclin dependent kinase 4; cyclin dependent kinase 6; cyclin dependent kinase inhibitor 1; cyclin dependent kinase inhibitor 1B; plant medicinal product; plumbagin; retinoblastoma protein; taiwanin E; unclassified drug; cyclin D1; cyclin dependent kinase; cyclin dependent kinase inhibitor 1A; growth inhibitor; plant extract; retinoblastoma protein; antiproliferative activity; Article; cancer growth; cancer inhibition; cell cycle checkpoint; cell cycle G0 phase; cell cycle G1 phase; cell cycle progression; concentration response; controlled study; drug cytotoxicity; drug isolation; Eleutherococcus; Eleutherococcus trifoliatus; human; human cell; IC50; MCF 7 cell line; protein expression; protein phosphorylation; Taiwan; breast tumor; cell cycle; cell proliferation; chemistry; down regulation; drug effects; female; genetics; MCF 7 cell line; metabolism; pathophysiology; phosphorylation; Breast Neoplasms; Cell Cycle; Cell Proliferation; Cyclin D1; Cyclin-Dependent Kinase Inhibitor p21; Cyclin-Dependent Kinases; Down-Regulation; Eleutherococcus; Female; Growth Inhibitors; Humans; MCF-7 Cells; Phosphorylation; Plant Extracts; Retinoblastoma Protein
Type
journal article