The effect of galectin 1 on 3T3 cell proliferation on chitosan membranes
Journal
Biomaterials
Journal Volume
25
Journal Issue
17
Pages
3603-3611
Date Issued
2004
Author(s)
Abstract
Galectin-1 (GAL1), a β-galactoside-binding protein, functions in cell adhesion, development, and growth regulation. A number of studies suggest that GAL1 play an important role in enhancing cell adhesion to extracellular matrix and inducing cell proliferation. Chitosan is a derivative of chitin extracted from lobsters, crabs and shrimps' exoskeletons. In clinical medicine, chitosan membrane had been used as a semi-permeable biological dressing. Although chitosan membranes show no cytotoxicity, some cell types (e.g. 3T3 cells) fail to attach and proliferate on their surface. In these studies, we show that over-expression of GAL1 does not enhance 3T3 cell proliferation on chitosan membranes. However, coating the chitosan membrane with recombinant GAL1 proteins significantly expedites 3T3 cells proliferation. The enhanced cell growth was inhibited by thiodigalactoside (TDG, a potent inhibitor of β-galactoside binding) and GAL1 monoclonal antibodies, suggesting GAL1's specific effect on the proliferation of 3T3 cells upon chitosan membranes. Moreover, immunoblotting detected a markedly suppressed tyrosine phosphorylation in several proteins on 3T3 cell growths upon GAL1-coated chitosan membrane. Pretreating the cells with sodium fluoride (NaF, a phosphatase inhibitor) inhibits the attachment and proliferation of 3T3 cells. These findings support a proposed role for altered levels of protein phosphorylation in GAL1-mediated cell attachment and proliferation on chitosan membranes. ? 2003 Elsevier Ltd. All rights reserved.
Subjects
Chitin; Chitosan membrane; Galectin; Phosphotyrosine
Other Subjects
Adhesion; Antibodies; Cell membranes; Cytology; Enzymes; Immunology; Toxicity; Phosphorylation; Biomaterials; chitosan; fluoride sodium; galectin 1; monoclonal antibody; recombinant protein; thioglycoside; animal cell; article; artificial membrane; cell growth; cell proliferation; controlled study; drug effect; embryo; human; human cell; immunoblotting; mouse; nonhuman; priority journal; protein analysis; protein expression; protein phosphorylation; 3T3 Cells; Animals; Biological Dressings; Cell Division; Cell Size; Chitin; Chitosan; Coated Materials, Biocompatible; Dose-Response Relationship, Drug; Galectin 1; Materials Testing; Membranes, Artificial; Mice; Decapoda (Crustacea); lobster
Type
journal article