Enhanced bone tissue regeneration by porous gelatin composites loaded with the Chinese herbal decoction Danggui Buxue Tang
Journal
PLoS ONE
Journal Volume
10
Journal Issue
6
Date Issued
2015
Author(s)
Abstract
Danggui Buxue Tang (DBT) is a traditional Chinese herbal decoction containing Radix Astragali and Radix Angelicae sinensis. Pharmacological results indicate that DBT can stimulate bone cell proliferation and differentiation. The aim of the study was to investigate the efficacy of adding DBT to bone substitutes on bone regeneration following bone injury. DBT was incorporated into porous composites (GGT) made from genipin-crosslinked gelatin and β-triclacium phosphates as bone substitutes (GGTDBT). The biological response of mouse calvarial bone to these composites was evaluated by in vivo imaging systems (IVIS), micro-computed tomography (micro-CT), and histology analysis. IVIS images revealed a stronger fluorescent signal in GGTDBT-treated defect than in GGT-treated defect at 8 weeks after implantation. Micro-CT analysis demonstrated that the level of repair from week 4 to 8 increased from 42.1% to 71.2% at the sites treated with GGTDBT, while that increased from 33.2% to 54.1% at GGT-treated sites. These findings suggest that the GGTDBT stimulates the innate regenerative capacity of bone, supporting their use in bone tissue regeneration. ? 2015 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
SDGs
Other Subjects
beta tricalcium; calcium; dangui buxue tang; gelatin; genipin; unclassified drug; wound healing promoting agent; angelicae sinensis extract; biomaterial; danggui buxue decoction; gelatin; herbaceous agent; Huang Qi; adult; animal experiment; animal model; animal tissue; Article; biocompatibility; bone injury; bone prosthesis; bone regeneration; bone remodeling; controlled study; drug effect; drug response; histology; image analysis; imaging system; in vivo study; male; micro-computed tomography; mouse; nonhuman; porosity; rat; signal detection; skull injury; tissue regeneration; Angelica sinensis; animal; Astragalus (plant); bone; bone regeneration; cell differentiation; cell proliferation; chemistry; cytology; drug effects; Fractures, Bone; Institute for Cancer Research mouse; metabolism; plant root; Angelica sinensis; Animals; Astragalus Plant; Biocompatible Materials; Bone and Bones; Bone Regeneration; Cell Differentiation; Cell Proliferation; Drugs, Chinese Herbal; Fractures, Bone; Gelatin; Mice; Mice, Inbred ICR; Plant Roots
Type
journal article