T-DNA的嵌入干擾At3g22970基因表現且使阿拉伯芥的葉部形態異常
The T-DNA insertion interferes with At3g22970 gene expression to confer the abnormal leaf morphology in Arabidopsis thaliana
Date Issued
2007
Date
2007
Author(s)
Shiue, Shin-Huei
DOI
en-US
Abstract
The frontier of leaf morphogenesis is still undiscovered, particularly in the dicotyledonous plants. Therefore, using a T-DNA tagging vector, pPZP202: SK: BAR, to generate mutant pools. Searching for plants with abnormal leaves phenotype and identify the genes which influenced by T-DNA in Arabidopsis thaliana. In previous study, we have identified a curly leaf mutant, ‘R1-2’. T-DNA inserts in 2.5-Kbp upstream of the At3g22970 and At3g22970 gene expression was down-regulated in the R1-2. In this study, we focused on how and why T-DNA insertion interfered with At3g22970 gene expression in R1-2 to result the phenotype. At3g22970 showed a circadian rhythm expression and the most expression during the subjective dusk. The transcripts level of At3g22970 in R1-2 presented all down-expression and had a slight circadian rhythm expression that was compared to WT. We propose that T-DNA insertion may disrupt the function of some enhancer for promoting At3g22970 gene expression in R1-2. At3g22970 encodes a protein containing a highly conserved DUF506 domain (Domain of Unknown Function 506) and translocated into plastid; however function still unknown. Meanwhile, there are 14 genes which containing DUF506 domain and all shown unknown function in A. thaliana. Moreover, At3g22970 promoter-GUS assay showed that At3g22970 mainly expressed in younger tissues. Overexpression of At3g22970 shown different degree interfere with leaf morphology suggested that At3g22970 function with dosage-effect. Furthermore, yeast two-hybrid results showed that At3g22970 interacts with 9 different proteins which involved in photosynthesis, carbohydrate metabolism, signal transduction and translational regulation. The relationships between At3g22970 and the interacting proteins need further study to understand the physiological function of At3g22970 in the future.
Subjects
捲曲葉形
curly leaf
circadian rhythm
DUF506
Type
other
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