Application of Silk Fibroin/Tendon-Derived Extracellular Matrix Scaffold for Tendon Repair
Date Issued
2014
Date
2014
Author(s)
Lee, Jia-Ni
Abstract
Tendons, which play an important role in force transmission, are composed of densely packed collagen fibers. When tendons are injured, surgical treatments are used to repair the damaged tendon by autografts, allografts, xenografts, or prosthetic devices. However, the risks of donor-site morbidity, transplant rejection, and injury recurrence usually limit long-term function recovery. There is a need to engineer biomaterial scaffolds that mimic the native tendon to overcome these problems.
Previous studies have shown a lot of tendon extracellular matrix components as well as growth factors are still retained in decellularized tissues. Hence, we developed the silk fibroin/tendon-derived extracellular matrix (SF/t-ECM) scaffolds that are to be morphologically, biochemically, and histologically similar to the ECM of native tendon tissues.
The results indicate cell proliferation on the SF/t-ECM scaffolds was significantly higher than that on the silk fibroin (SF) control groups after 7 days of cell culture. Histology demonstrated cells infiltrated through the full thickness of the SF/t-ECM scaffolds. Moreover, in rat tendon repair model, the SF/t-ECM scaffolds exhibited more fibroblasts proliferation and collagen fibers synthesis as compared with the SF control groups after 16 days of scaffold implanted. This investigation demonstrate that SF/t-ECM scaffolds have potential application in tendon repair.
Subjects
肌腱組織工程
氣隙電紡絲法
蠶絲蛋白
細胞外基質
肌腱修復
Type
thesis
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